RNA Sequencing of Single Myofibers from Mus musculus

RNA-sequencing from single nuclei.

It has recently been established that synthesis of double-stranded cDNA can be done from a single cell for use in DNA sequencing. Global gene expression can be quantified from the number of reads mapping to each gene, and mutations and mRNA splicing variants determined from the sequence reads. Here we demonstrate that this method of transcriptomic analysis can be done using the extremely low le...

Leptospirosis in small mammals of Iran: II: Isolation of Leptospira grippotyphosa, from Mus musculus

Expanding the genetic code of Mus musculus

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal...

Ontogenesis of serum esterases in Mus musculus.

INTRODUCTION Electrophoretic techniques by which mixtures of isoenzymes, and homologous proteins generally, may be resolved have become valuable tools in developmental biology. They make possible the study of development at the molecular level, in the sense that the differentiation of protein patterns underlies ontogenetic changes and tissue differences. The greater successes of this approach h...

Gastrulation in Mus musculus (common house mouse)

As mice embryos develop, they undergo a stage of development called gastrulation [7]. The hallmark of vertebrate gastrulation [7] is the reorganization of the inner cell mass [8] (ICM) into the three germ layers [9]: ectoderm [10], mesoderm [11], and endoderm [12]. Mammalian embryogenesis [13] occurs within organisms; therefore, gastrulation [7] was originally described in species with easily o...